Annexin V‑FITC/PI staining (BD Biosciences) and flow cytometry quantified apoptosis after 48 h treatment (IC₅₀ concentration). Propidium iodide staining after ethanol fixation assessed cell‑cycle distribution.
The title refers to "off-paco," a Japanese slang term for sexual encounters arranged through social media or offline meetups. mukd-546
The production features a mix of established and amateur talent. Key performers credited in various database entries include: Himari Aizuki Minami Maeda Momo Shiraishi Availability and Formats The production features a mix of established and
The film’s narrative centers on a "casual hookup" or "off-party" scenario where a group of men and women meet with the intent to explore uninhibited sexual desires. The plot emphasizes the transition from the tension of an initial meeting to a wild, collaborative environment. Key thematic elements include: Key thematic elements include: Mice were randomized (Day
Mice were randomized (Day 7, when tumors reached ~100 mm³) to receive either vehicle (0.5 % methylcellulose) or Mukd‑546 (30 mg·kg⁻¹, oral gavage, QD) for 21 days. Tumor volumes measured twice weekly (V = ½ × length × width²). Body weight and clinical signs monitored. At endpoint, tumors were harvested for immunohistochemistry (IHC) of p‑ERK and Ki‑67.