The is a specialized tool (available as online web apps, Excel spreadsheets, or embedded in chromatography data systems) designed to predict the geometrical and operational parameters needed to make two different columns produce equivalent chromatographic results .
Moving from analytical (4.6 mm ID) to semi-prep (10 mm ID) requires a flow rate increase by a factor of ~4.7. The calculator does this instantly, preventing solvent waste and poor peak shape. hplc column equivalent calculator