| Feature | GraphPad Prism | Excel | R/Python | |---------|----------------|-------|-----------| | One‑click t test | ✅ | ✅ (Analysis ToolPak) | ❌ (requires code) | | Normality test | ✅ | ❌ | ✅ | | Effect size & CI | ✅ | ❌ (CI manual) | ✅ | | Publication graph | ✅ (built‑in) | ⚠️ (basic) | ✅ (requires plotting library) | | Cost | Paid | Often free | Free |
Find the "Analyze" button in the toolbar and select "t tests (and nonparametric tests)" from the Column Analysis list. Configure the Analysis: Choose between paired or unpaired. Decide if you assume a Gaussian (normal) distribution. graphpad t test
Compares means from the same subjects at different times (e.g., Before vs. After treatment). | Feature | GraphPad Prism | Excel |