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Depth of field is the area of the image that appears acceptably sharp. In macro photography, DoF is razor-thin. At 1:1 magnification and an aperture of f/16, the depth of field may be mere millimeters.
As magnification increases, the effective aperture decreases, resulting in light loss. For example, at 1:1 magnification, a lens set to f/2.8 effectively behaves like an f/5.6 lens in terms of light transmission. This necessitates longer shutter speeds or higher ISO settings, which introduce noise and motion blur risks. makro best
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