The QIAGEN PE Buffer typically consists of:
He stared at the bottle. "Is that it?"
He pulled up a stool and began to write the recipe—the forbidden recipe that turns a dependent grad student into a master of their domain.
: Tick the box on the bottle cap to indicate that ethanol has been added. Homemade Alternative (5X Stock)
He double-checked his mental math. 10x dilution of the Tris stock... high ethanol content... pH around 8. It was a simple wash buffer. It was a harsh solvent for proteins, a dehydrating agent for the salts, but gentle enough not to strip the DNA from the silica.
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