Pgps3 Unique Restriction Sites ★ [SAFE]

PGPS3’s set of unique restriction sites——gives you a versatile toolkit for plant expression vectors. The small backbone (3.2 kb) means your total construct size stays under the 10–12 kb transformation efficiency sweet spot for Agrobacterium .

The most common workflow for PGPS3 is inserting a gene under the control of the CaMV 35S promoter (which is already in the vector upstream of the MCS). pgps3 unique restriction sites

: When sequencing from the transposon ends to identify insertion sites, unique restriction sites nearby serve as reliable reference points for mapping the relative position of the "hop" into target DNA. PGPS3’s set of unique restriction sites——gives you a

These sites are often used for linearization or diagnostic digests. : When sequencing from the transposon ends to

If you want to replace the 35S promoter with a tissue-specific promoter (e.g., RBCS or AtUBQ10 ):

) marker. This marker can be removed or replaced using unique restriction sites that flank the gene, or by using or EcoRV , which also interact with the Transprimer region.

) into the pGPS3 vector to create custom transposon constructs.