| Metric | Manual (expert) | ITCN (optimized) | Analyze Particles | |--------|----------------|------------------|--------------------| | Time per image | 3–5 min | 3–5 sec | 2 sec | | Accuracy vs. manual | – | 94–97% | 62–78% (fails on clusters) | | Repeatability (CV, n=5) | 4–8% | 1–2% | 15–30% | | Handling of clusters | Excellent | Good (width tuning) | Poor |
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: Close and reopen ImageJ. The tool will appear under the Plugins menu (often specifically Plugins > Filters > ITCN ). Step-by-Step Usage Guide itcn imagej plugin
: The plugin allows users to interactively adjust thresholding parameters to segment nuclei from the background or from other cellular structures. This is crucial for obtaining accurate counts and for analyzing the morphology of nuclei. | Metric | Manual (expert) | ITCN (optimized)