If you provide your (e.g., phase contrast, DIV7 cortical neurons, 40x) and whether you need total neurite length per cell or average neurite length , I can tailor the macro or tracing settings further.
Draw a precise line along the entire length of your embedded scale bar. Navigate to . neurite length measurement imagej
// Batch neurite length measurement - skeletonization method setBatchMode(true); dir = getDirectory("Choose source directory"); output = getDirectory("Choose output directory"); list = getFileList(dir); for (i=0; i<list.length; i++) if(endsWith(list[i], ".tif")) open(dir + list[i]); // Preprocessing run("8-bit"); run("Subtract Background", "rolling=50"); run("Enhance Contrast", "saturated=0.35"); setAutoThreshold("Default"); run("Convert to Mask"); run("Skeletonize"); // Measure skeleton run("Analyze Skeleton (2D/3D)"); saveAs("Results", output + list[i] + "_skeleton.csv"); close(); If you provide your (e
Check if you wish to apply this identical scale to all sequential images from the same imaging session. Click OK . // Batch neurite length measurement - skeletonization method
Install these via :
